Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma / 华西口腔医学杂志

OBJECTIVE@#To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).@*METHODS@#Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.@*RESULTS@#Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.@*CONCLUSIONS@#Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.

Expression of large tumor suppressor homolog 2 gene and its promotor methylation in oral squamous cell carcinoma / 中华口腔医学杂志

Objective@#To investigate the expression of large tumor suppressor homolog 2 (LATS2) gene and its promotor methylation in oral squamous cell carcinoma (OSCC).@*Methods@#Reverse transcription-PCR (RT-PCR) and pyrosequencing were used to detect the mRNA and promotor methylation of LATS2 gene in 72 OSCC specimens and normal oral mucosa tissues. Western blotting was used to detect the LATS2 protein in six OSCC specimens and normal oral mucosa tissues.@*Results@#All cases had expression of LATS2 mRNA in normal oral mucosa tissues, but the expression was down-regulated significantly, only 47% (34/72) in 72 cases of OSCC showed LATS2 mRNA expression. The expression was correlated with the degree of tumor differentiation and lymph node metastasis (P<0.05). The results of pyrosequencing show that 68% of promotor methylation (49/72) in 72 cases of OSCC. Furthermore, there was significant correlation between the mRNA and promotor methylation of LATS2 gene (χ2=16.980, P<0.01). All the six specimens had the low LATS2 protein expression.@*Conclusions@#The promotor methylation of LATS2 gene may play an important role in the occurrence of OSCC.

Correlation of LATS2 mRNA and protein expression in oral squamous cell carcinoma / 实用口腔医学杂志

RT-PCR and Western blot were used to detect the LATS2 mRNA and protein level in 57 cases of oral squamous cell carcinoma (OSCC)specimens and 12 normal oral mucosa tissues.LATS2 mRNA and protein were expressed in all the normal oral mucosa samples (100%),but only 47.4% (27 /57)and 42.1% (24 /57)in OSCC samples respectively with positive correlation of both(P <0.01),and were respectively correlated with the tumor differentiation degree and lymph node metastasis(P <0.05).

Results analysis of serum alanine transaminase measurement in 23 clinical laboratories with target value assigned by reference method / 中华检验医学杂志

Objective To explore the feasibility of inter-laboratory comparison and trueness evaluation among clinical laboratories, and assess the quality of participants′measurement, by measuring the activity of alanine aminotransferase ( ALT ) in patient serum samples.Methods Method comparison study was used.Five patients serum samples, whose target values were assigned by two international candidate reference laboratory with reference method of ALT without pyridoxal phosphate, were measured by 23 routine laboratories.The bias between measurement result of each participant and the mean of reference laboratories was calculated, and then compared to allowable bias 6%.Calculate the mean value and the relative bias.Results Compared with the mean of reference laboratories, the maximum absolute value of bias among the 23 routine laboratories was 31.27%.The rate range which bias was less than the allowable bias was 26.09%-73.91 %.The bias acceptability of 8 participants were more than or equal to 80%;15 participants were less than or equal to 60%; and 3 participants were 0%.Conclusions Using patient serum samples and values assigned by reference method is an effective way to carry out inter-laboratory comparison and trueness evaluation.It can reflect the quality of measurement more truly.

Detection of small supernumerary marker chromosome by single nucleotide polymorphism microarray chips / 中华儿科杂志

<p><b>OBJECTIVE</b>To apply single nucleotide polymorphism (SNP) microarray for delineation of small supernumerary marker chromosome (sSMC) in two newborns.</p><p><b>METHOD</b>Chromosome karyotyping was performed on newborns who were born in Jan. 2013 and Jan. 2014 in Haidian Maternal and Child Health Hospital because of the abnormalities found in pregnancy checkups. SNP microarray analysis was carried out on 2 newborns with de novo sSMCs (one was mos 47,XY, + mar[45]/46,XY[5] and the other was mos 47, XY, + mar [30]/46, XY [20]), which could not be determined by conventional banding techniques. Genomic DNA was extracted from cord blood samples, amplified, tagged and hybridized following the manufacturer' s protocol. Data were collected and analyzed.</p><p><b>RESULT</b>There was a 78. 6 Mb duplication in chromosome 8 for Newborn A, which was associated with 8p22 duplication syndrome; and a 32. 7 Mb duplication in chromosome 13 for Newborn B, which was not yet reported definitely as pathogenic. The newborn A was identified with agenesis of the corpus callosum, obvious right eyelid drooping, the onset of low muscle tone and mental developmental lag behind their peers, while the newborn B had normal findings on physical and mental evaluation.</p><p><b>CONCLUSION</b>SNP-array can identify sSMCs of newborns at the DNA level, and can be used as an important supplement to the conventional karyotype analysis, but the pathogenicity of positive outputs need further verification.</p>

Serological response infected with on cynomolgus monkeys / 中国免疫学杂志

Objective:To study the serological reactions in cynomolgus monkeys infected with hepatitis B virus ( HBV). Methods:To select 1 to 3 days old or adult healthy cynomolgus monkeys by artificial breeding to observe the virology screening in laboratory a month to confirmed healthy animals ,randomly divided into control group and infection group .Infection group vaccination serum HBV carriers 0.5 ml (HBV-DNA≥108 copies) single cages,observe each group behavioral changes daily after inoculation 1 to 12 weeks, each week to confirmed the degree of liver inflammation through the HBV-M, HBV-DNA, liver function and on the B-guided, liver tissue inflammation by routine HE staining .Results: Adult monkeys did not induce positive reaction after vaccination , there were three young monkeys appear HBsAg , HBcAb and 2 appear HBV-DNA reaction, ALT poison attack occurred in HBsAg-positive began to increase after one week , one month after the peak , which was 180 U/L, after gradually decreased , continuing a month later near normal .AST higher than a week after the normal reference values were flat curve , representing the peak ALT after a month later, HBsAg positive cynomolgus monkeys HE staining showed mild hepatitis partial liver tissue lesions .Conclusion:HBV-M, HBV-DNA, ALT, AST and liver histopathology after HBV infection have changed , this result showing that it's produce inflammation and induction the response of immune .

Clinicopathologic features and differential diagnosis of multilocular cystic renal cell carcinoma / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathological characteristics and the diagnosis of multilocular cystic renal cell carcinoma (MCRCC).</p><p><b>METHODS</b>The clinicopathological data of 19 MCRCC cases were collected and immunohistochemical staining assays were carried out. Forty-six cases of other cystic kidney lesions within the same period were collected as controls, including extensively cystic clear cell RCC (12 cases), clear cell tubulopapillary renal cell carcinoma (6 cases), tubulocystic carcinoma (2 cases), simple cortical cysts (22 cases), multilocular cystic nephroma (1 cases) and multicystic kidney (3 cases).</p><p><b>RESULTS</b>The patients included 14 males and 5 females. The ages ranged from 31 to 66 years (median age = 50 years). Most of the MCRCC cases were detected incidentally in physical examination, occasionally accompanied with hematuria, back pain or other symptoms. The follow-up period of 17 patients ranged from 6 to 170 months. All patients were alive without evidence of tumor recurrence or metastasis. Pathological findings showed that macroscopically, tumor size ranges from 1.5 to 7.0 cm in the maximum diameter, generally a entirely of various sized. The cysts contain serous, hemorrhagic or turbid fluid. Solid areas or substantially discernible mural nodules were absent; histologicallly, single layer of cuboidal and flattened epithelial tumor cells were lined in the cysts, described as clear cytoplasm, small nuclear, no nucleoli and low Fuhrman nuclear grade (I or II). Multilayer tumor cells could be observed in a few cysts, with granular cytoplasm and small intracystic papillae formed. The clear tumor cell clusters, similar as cystic lined tumor cells, were seen within pathological fibrous in almost all cases, and significant myofibroblastic proliferation was found in 14 cases. Immunohistochemically, the cysts lined epithelial cells and the clear tumor cell clusters were positive for epithelium markers, including CKpan(19/19), EMA(16/19) and CK7 (15/19); higher percentage of CAIX (17/19) and PAX8(15/19) than control groups, but lower percentage of CD10 (7/19), RCC (6/19) and AMACR(2/19); and all were negative for 34βE12, CD117 and CD68.</p><p><b>CONCLUSIONS</b>Multilocular cysts, clear cells clusters of low Fuhrman grade within fibrous septa and capillary vessel proliferation under epithelium are important features of MCRCC. The united using of CAIX, CK7, CD10 and RCC is helpful for differentiating variable cystic renal tumor. MCRCC usually has an excellent prognosis, nephron sparing surgery is first recommended as a therapeutic strategy.</p>

Laparoscopic-assisted transanal Soave pull-through procedure for Hirschsprung's disease and allied disorders / 中华普通外科杂志

ObjectiveTo explore the feasibility and outcomes of natural orifice transanal laparoscopic Soave procedure for Hirschsprung's disease and allied disorders (HAD).MethodsFrom March 2010 to December 2011,31 cases (at the age from 3 mos to 6 yrs) with Hirschsprung's disease or allied disorders (5 cases)underwent laparoscopic-assisted Soave pull-through procedure at two tertiary hospitals.We modified this technique by mobilizing the left hemicolon or whole colon via rectal muscular sleeve approach under transanal or transumbilical laparoscopic vision,then endorectal pull-through to completearectosigmoidectomyorsubtotalcolectomy. ResultsAllprocedureswerecompleted successfully.A rectosigmoidectomy was performed in 16 cases with classic HD and subtotal colectomy in 15 cases with extended HD and HAD.The average operative time was ( 117 ± 13) min.The length of the resected segment was 35 -80 cm,and the estimated blood loss was 5 -20 ml. One infant developed postoperative intestinal obstruction that required open exploration.Follow-up of one to 20 mos found no stoma stenosis or constipation recurrence. Enterocolitis developed in 1patient.ConclusionsTransanal or transumbilical laparoscopic-assisted Soave pull-through surgery is safe,effective and with a benefit of much less invasion and almost invisible scars.

Laparoscopic surgery for complex choledochal cysts / 中华普通外科杂志

Objective To summarize our experience of laparoscopic surgery for complex choledochal cysts (type Ⅳ-A). Methods The clinical data of 65 children of choledochal cyst undergoing laparoscopic choledochal cyst resection were retrospectively reviewed from 2002 to 2009 in our institute.Among those type Ⅳ-A cyst was found in 16 patients. Hepaticojejunostomy was performed using a Roux-en-Y jejunal loop after extrahepatic cyst excision and ductoplasty. Results Laparoscopic procedures were successfully performed in 16 patients with type Ⅳ-A cysts. The stenotic segment was splited or excised and a wide hepaticojejunostomy was completed at the porta hepatis in 8 patients with a stricture extending to the level of common hepatic duct. The constrictive confluence of the bilateral hepatic duct was incised and the bi-ductal cystojejunostomy was achieved at the bifurcation in 4 cases. A septum was found at the orifice of right hepatic duct and was excised through the hilar stoma in 2 cases. A downstream stricture of the left hepatic duct was incised from the hilum to the dilated segment along the lateral wall in 2 patients, so that a long intrahepatic cystojejunostomy was completed in an oblique course. Postoperative complications developed in 2 cases including temporary bile leakage in one case and anastomotic stricture in another. The intrahepatic cysts were remarkably reduced in size during the follow-up. Conclusions With the magnified laparoscopic view, the radical resection of extrahepatic cyst and correction of the intrahepatic bile ductal stenosis can be easily performed. Laparoscopic hepaticojejunostomy and/or intrahepatic cystojejunostomy is effective and safe for children with type Ⅳ-A choledochal cysts.

Splenic vessels thread ligature in laparoscopic splenectomy / 中华普通外科杂志

Objective To study the anatomy of splenic hilum blood vessels in order to thread ligature(endoligature)instead of using stapler during the process of laparoscopic splenectomy and to evaluate the prelimnary clinical results.Methods 41 children patients underwent laparoscopic splenectomy with this technique(endoligature)for various hematologic and autoimmune disorders,including 25 cases of hereditary spherocytosis,13 idiopathic thrombocytopenia purpura,and 3 hypersplenic granulocytopenia.The anatomy of splenic pedicle,the adjacent relation between splenic vessel and pancreas were detected by color Doppler ultrasonography preoperatively.The above-mentioned parameters were compared with that found intraoperatively.Results The relationships of splenic vessel and pancreas was of type Ⅰ in 24 cases and type Ⅱ in 17.In 31 cases,the major splenic blood vessels were ramified into branches 2 cm away from the hilum and in 10 it was within 2 cm as detected by preoperative ultrasonography.These characters were largerly identified by laparoscopic laparotomy,and in all the 41 cases laparoscopic splenectomy was successfully accomplished using this endoligature instead of vasculature stapler.There was no serious complication.The mean operating time was(114 ±31)min,the estimated blood loss was(51 ±23)ml.Conclusions Ultrasonography could identify the anatomic type of splenic vessel,and its relation with the pancreas.Endoligature in the management of splenic pedicles during laparoscopic splenectomy is safe and reliable.

The expression and significance of RUNX3 gene in hepatocellular carcinoma / 中国医师进修杂志

Objective To evaluatethe expression and significance of RUNX3 gene and protein in hepatocellular carcinoma.Method Reverse transcription-polymerase chain resction (RT-PCR) and immunoitistochemistry SP method were used to detect the expressions of RUNX3 gene and protein in 23 cases of hepatocellular carcinoma and their adjacent non-cancerous tissue.Results The expression of RUNX3 gene and protein were significantly reduced in hepatocellular carcinoma tissue (26.1% and 17.4% respectively),which were prominently lower than those in adjacent non-cancerous tissue:91.3% (21/23) and 87.0% (20/23),P＜0.01.Conclusion The lower-expression of RUNX3 gene and protein may play an important role in hepatocellular carcinoma.

Surveillance of Antimicrobial Resistance of Klebsiella pneumoniae in Nosocomial Infections During 1999-2004 / 中华医院感染学杂志

OBJECTIVE A surveillance study was performed for nosocomial infections in order to investigate the change in antimicrobial resistance of Klebsiella pneumoniae,especially the strains isolated from 1999 to 2004.METHODS K-B test was used for the antibiotics susceptibility test and the results were read based on National Committee for Clinical Laboratory Standards(NCCLS) of the USA.The situation of ESBLs-producing strains of K.pneumoniae was investigated.RESULTS Totally 326 K.pneumoniae strains showed the highest susceptibility to imipenem.Ceftazidime,cefepime,and cefoperazone/sulbactam also showed excellent activity against K.pneumoniae.The prevalence of ESBLs from 326 strains was 20.2%.CONCLUSIONS It is important to study the drug resistance in nosocomial infections by K.pneumoniae.

Expression of connective tissue growth factor is enhanced in human and rat liver fibrotic tissues / 基础医学与临床

Objective To explore the role of connective tissue growth factor(CTGF)in the mechanism of liver fibrosis.Methods Liver fibrosis models of rats were made by subcutaneously injecting with CCl4.On the other hand,32 human liver cirrhosis and 12 normal liver tissues were collected too.Immunohistochemical staining was used to detect the expression of CTGF in these tissues.Results The expression of CTGF in rats after injection with CCl4 was found mainly in hepatic stellate cells(HSC) and hepatocytes are all significantly higher than those in normal rats.Moreover,it presents with progressive tendency for the expression of CTGF in rats respectively at 1st,4th and 8th week after injection of CCl4.The same tendency was found in human normal and fibrotic liver tissues(P

The Effect of Helicobacter Pylori Eradication on Atrophy of Gastric Antrum Mucosa and Intestinal Epithelium Mucosae Metaplasia / 中国慢性病预防与控制

Objective To investigate the pathological changes of gastric antrum mucosa after Helicobacter pylori eradication.Methods Total 180 cases who suffered from epigastralgia and took the endoscopic examination were randomly divided into two groups,one was Hp eradication group which included 98 cases and given anti-Hp medication treatment,and the other was control group which included 82 cases and given an expectant treatment.At the end of the study,they took reexamination by gastroscope and tests on Hp by Giemsa dyeing & rapid urase detection and on gastric antrum pathological changes by HE dyeing.Results In treatment group,atrophic gastritis as well as intestinal metaplasia decreased significantly after Helicobacter pylori eradication,but in control group,no change of atrophic gastritis was found while intestinal metaplasia aggravated.Conclusion The eradication of Helicobacter pylori is able to decrease atrophic gastritis and intestinal metaplasia.

Laparoscopic sutured gastrojejunostomy without using stapling devices in children / 中国微创外科杂志

Objective To explore the method, safety, and efficacy of laparoscopic intracorporeal sutured gastrojejunostomy.Methods Three children with gastric outlet obstruction underwent laparoscopic sutured gastrojejunostomy from May to September 2005. Of them, two patients with pyloric stenosis secondary to peptic ulceration received a concomitant highly selective vagotomy. A suitable segment of jejunum was lifted over the transverse colon and apposed to the gastric antrum. A continuous 4/0 suture was conducted at the seromuscular layer making the two organs together. Then the stomach and adjacent jejunum were incised with an ultrasonic scalpel. A side-to-side gastrojejunostomy was performed with full-thickness continuous suture of gastric and jejunal wall followed by anterior interrupted suture of seromuscular layers. Results All the intracorporeal sutured gastrojejunostomies were completed successfully under laparoscope. The operating time was 135, 150, and 180 min, respectively. The postoperative hospital stay was 6 d. There was no surgical complications. Postoperative follow-up at 8, 10, and 12 months, respectively, showed that all patients had normal diet and nutriture. Conclusions Laparoscopic sutured gastrojejunostomy is a safe and feasible technique, with advantages of minimal invasion, rapid recovery, and good cosmetic outcomes.

Development and application of multi-function joint device for oxygen supply by endotracheal tube / 医疗卫生装备

Objective To solve the problem of additional airway resistance from oxygen supply tube and humidity liquid dropping tube in trachea pipe for patients with trachea dissection and intubatton along with the fixation of respiration detector.Methods The device was used in patients with trachea dissection and intubatton and the result is desirable.Conclusion This device solves the problem of additional airway resistance from oxygen supply tube and humidity liquid dropping tube in trachea pipe along with the fixation of respiration detector.It is an indispensable device for patients with trachea dissection and intubatton.

Expression of extracellular signal-regulated kinase in liver fibrotic tissues of human / 中国实用内科杂志

Objective To explore the role of Extracellular signal-regulated kinase(ERK)signal transduction pathway in the mechanism of liver fibrosis via investigating the expression of ERK1 in fibrotic liver tissue of human.Methods SABC immunohistochemistry was used to detect the expression of ERK1 in 32 liver fibrosis and 12 normal liver tissues from Jan.2001 to Dec.2003.Results The expression of ERK1 in liver fibrotic tissues were found chiefly in hepatic stellate cells(HSC),and it presented with significantly progressive tendency for the expression of ERK1 in normal and liver fibrosis cirrhosis tissues(P

Laparoscopic Ladd’s procedure for intestinal malrotation in children / 中国微创外科杂志

Objective To explore the feasibility of laparoscopic Ladd’s procedure and its indications for intestinal malrotation accompanying midgut volvulus. Methods Laparoscopic Ladd’s procedure was performed in 15 children from July 2002 to March 2006. The procedure was performed using three trocars. Under laparoscopic visualization, the midgut volvulus was untwisted by grasping and pulling the intestine, the Ladd’s band was divided and broadened, the duodenum and the small intestine were mobilized, and finally an appendectomy was performed through an abdominal wall port. Results Laparoscopic Ladd’s procedure was completed successfully in the 15 children. Of them, 1 patient with duodenal web, 1 patient with paraduodenal hernia, and 1 patient with ectopic pancreas also had a concomitant procedure. The operative time was 45~150 min (mean, 75 min). The patients began to take food on 1~3 postoperative day. There was no surgical complications. The length of postoperative hospital stay ranged 4~6 days (mean, 5 days). Follow-up observations in 12 patients for 1~42 months (mean, 21 months) showed normal development and free of symptoms.Conclusions Laparoscopic Ladd’s procedure is a safe and effective technique. It can be performed in neonates and applicable subacute midgut volvulus, but may be unadvisable for acute volvulus with abdominal distention.

Laparoscopic massive splenectomy combined with selective pericardial devascularization using endoligature technique / 中国微创外科杂志

Objective To summarize the experience of laparoscopic massive splenectomy combined with selective pericardial devascularization using endoligature technique. Methods By using silk ligature and hamonic scapel or the LigaSure, 6 patients with portal hypertension and esophagogastric varices underwent laparoscopic massive splenectomy and selective pericardial devascularization. Results All the operations were completed successfully under laparoscope. The intraoperative blood loss was 80~200 ml (mean, 130 ml). None of the patients required blood transfusion or conversion to open procedure. The operation time was 150~210 min (mean, 190 min). There were no surgical complications. All the patients resumed to normal activities 5 days after operation. Follow-up observations for 3~10 months (mean, 8 months) revealed no recurrent variceal hemorrhage. Conclusions Laparoscopic massive splenectomy combined with selective pericardial devascularization using endoligature technique is a feasible, effective, safe, little hemorrhagic, and minimally invasive procedure for portal hypertension with esophagogastric varices.